Petra Wierup. Foto: Pressbild“For it to be permitted to use a service provider in a third country (outside the EU), it is required that robust agreements with instructions are in place. It must also be ensured that there is legal support for the transfers, so that the data that is transferred receives continued strong and equivalent protection when it is processed in a third country. The protection must therefore not become weaker when it is processed by subcontractors”, says Petra Wierup.
This reaction would contain only low concentrations of the dNTPs and relied upon brief incubation times, so that DNA synthesis would stall at random positions along the template and yield a population of DNA fragments with varying lengths. These unfinished DNA fragments were then purified and used as templates in four “minus” and four “plus” reactions.
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