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Sequencing technologies are not perfect. They make mistakes, as we can see from the accuracy rates reported in Section 1.2. For technologies based on nucleic acid synthesis (i.e. everything except ONT), since they use polymerases it stands to reason that the same three types of errors, described in Section 1.1.2.2, occur: substitutions, insertions and deletions. For long read technologies though, most of the errors do not come from the polymerase, but from signal processing used to deduce the sequence. Since both technologies execute single molecule sequencing, the signal to noise ratio is low68,69 making base calling more complicated.
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